PYCC 9315

General Information

Taxonomy

1 - Taxon name

Torulaspora pahokee

2 - Classification

Ascomycota

3 - Strain (species name) changes

Torulaspora pretoriensis

4 - Status of the strain

5 - Basis for identification

Molecular (whole genome sequence)

6 - Identified by

Coughlan, Aisling Y., et al. 2020

Other Collections

7 - Original strain number

ML 4864; 9609-116

8 - Accession numbers in other collections

CBS 11124

Biosafety and restrictions

9 - Biological Safety Level

BSL-1

Strain details

11 - PYCC strain status

Open

12 - Mediterranean strain

13 - Substrate of isolation

sea water

14 - Category of substrate

Water

15 - Locality

Turkey Key Camp Site, Ten Thousand Island, Gulf of Mexico, Everglades National Park, Florida

16 - Country of origin

USA

18 - Sample Collected by

05/06/1998

19 - Isolated by and date of isolation

20 - Isolation details

21 - Deposited by

CBS, Jun. 2022

22 - History

J.W. Fell collection > CBS > PYCC

23 - Preservation

Glass beads; 20% Glycerol; -150C

24 - Price per culture

90€

25 - Remarks

Growth conditions

26 - Medium for growth

YMA

Molecular data

Whole genome

GCA_012851045.1

Phenotype

Fermentation

F1 D-Glucose

F2 D-Galactose

F3 Maltose

F5 Sucrose

W, D

F6 a,a-Trehalose

F7 Melibiose

F8 Lactose

F9 Cellobiose

F10 Melezitose

F11 Raffinose

Assimilation-Growth

C1 D-Glucose

C2 D-Galactose

C3 L-Sorbose

C4 D-Glucosamine

C5 D-Ribose

C6 D-Xylose

C7 L-Arabinose

C8 D-Arabinose

C9 L-Rhamnose

C10 Sucrose

C11 Maltose

C12 a,a-Trehalose

C13 Me a-D-Glucoside

C14 Cellobiose

C15 Salicin

C17 Melibiose

C18 Lactose

C19 Raffinose

C20 Melezitose

C21 Inulin

C22 Starch

C23 Glycerol

C24 Erythritol

C25 Ribitol

C26 Xylitol

C28 D-Glucitol

C29 D-Mannitol

C30 Galactitol

C31 myo-Inositol

C32 D-Glucono-1,5-lactone

C35 D-Gluconate

C36 D-Glucuronate

C38 DL-Lactate

C39 Succinate

C40 Citrate

C41 Methanol

C42 Ethanol

C50 L-Malic acid

C51 L-Tartaric acid

N1 Nitrate

N2 Nitrite

N3 Ethylamine

N4 L-Lysine

N5 Cadaverine

N6 Creatine

N7 Creatinine

V1 w/o vitamins

T1 Growth at 25ºC

T1 Growth at 30ºC

T3 Growth at 35ºC

T4 Growth at 37ºC

T5 Growth at 40ºC

T6 Growth at 42ºC

T7 Growth at 45ºC

O1 Cycloheximide 0.01%

O2 Cycloheximide 0.1%

P1 Protocatechuic acid

M1 Starch formation

M3 Urea hydrolysis

Bibliography

Title

A taxogenomic view of the genus Torulaspora: an expansion from ten to twenty-two species

Author

M.R. Silva, F. Paraíso, J. Al-Oboudi, M. Abegg, A. Aires, K.O. Barros, P.H. Brito, M. Jarzyna, K. Sylvester, Q.K. Langdon, D.A. Opulente, F. Carriconde, J.W. Fell, T.A. Hofmann, M.-A. Lachance, J.-L. Legras, D. Libkind, A. Pontes, P. Gonçalves, C.A. Rosa, M. Groenewald, C.T. Hittinger, J.P. Sampaio

Abstract

The yeast genus Torulaspora (subphylum Saccharomycotina, family Saccharomycetaceae) is mostly known from its type species, T. delbrueckii, a frequent colonizer of wine and sourdough bread fermentations. The genus currently contains 10 species that are typically found in various natural terrestrial environments in temperate and tropical climates. Here we employ taxogenomic analyses to investigate a large collection of Torulaspora strains obtained in multiple surveys we carried out in Asia, Australasia, North America, South America, and Europe, and to which we added several strains maintained in culture collections. Our analyses detected twelve novel species that are formally described here, thereby more than doubling the species diversity of Torulaspora. We also sketch a genotype-phenotype map for the genus and show how the complex relationship between key genes and the physiological traits they control both between and within species. This remarkable increase in the number of species in the genus Torulaspora highlights how limited the current inventory of fungal taxa is. It also shows how integrated taxogenomic approaches can foster the assessment of species circumscriptions in fungi.

Publication Date

26 May 2025

Link to Publication

https://doi.org/10.3114/persoonia.2025.54.08