PYCC 9021
1 - Taxon name
Kazachstania surinensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
Kazachstania sp.
4 - Status of the strain
Type strain of Kazachstania surinensis
5 - Basis for identification
Molecular (D1D2)
6 - Identified by
Nantana Srisuk
7 - Original strain number
SRFS57-2
8 - Accession numbers in other collections
TBRC 15053
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Fermented fish
14 - Category of substrate
Food & beverages
15 - Locality
Market, Mueang Surin District, Surin Province
16 - Country of origin
Thailand
18 - Sample Collected by
NA
19 - Isolated by and date of isolation
Sukrita Punyauppapath, Jan. 2021
20 - Isolation details
NA
21 - Deposited by
Sasitorn Jindamorakot, Nov. 2021
22 - History
Sasitorn Jindamorakot > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
Title
Kazachstania surinensis f.a., sp. nov., a novel yeast species isolated from Thai traditional fermented food

Author

Sukrita Punyauppa-path, Prasongsom Punyauppa-path, Suriya Tingthong, Varunya Sakpuntoon, Pannida Khunnamwong, Savitree Limtong, Nantana Srisuk

Abstract

Seven yeast strains (UBIF12-1, UBFB13-1, SRFS56-3, SRFS57-2, SKFS62-1, SKFS66-1 and SKFS67-1) representing a single anamorphic novel yeast species were isolated from traditional Thai fermented foods in Ubon Ratchathani, Surin and Sisaket in the northeast part of Thailand. The results of analysis of the sequences of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region indicated that the seven strains showed zero to one nucleotide substitutions in the sequences of the D1/D2 region of the LSU rRNA gene, and zero to four nucleotide substitutions in the ITS region. Kazachstania humilis CBS 5658T was the most closely-related species, but with 0.7–0.9% nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, and 2.0–2.2% nucleotide substitution in the ITS region. The results of a phylogenetic analysis based on the concatenated ITS and D1/D2 regions confirmed that the seven strains represented a single species of the genus Kazachstania distinct from the other recognized species of the genus. Furthermore, the morphological, biochemical and physiological properties of the seven strains not only indicated that they represented members of the genus Kazachstania, but that they were separated from K. humilis and K. pseudohumilis, the two most closely related species in the phylogenetic tree. Therefore, the seven strains were identified as representing a novel species, for which we propose the name Kazachstania surinensis f.a., sp. nov. The holotype is TBRC 15053T (isotype: SRFS57-2 and PYCC 9021). The MycoBank number of the novel species is 841892.

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.005488