PYCC 9020
1 - Taxon name
Exophiala amphawaensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Exophiala amphawaensis
5 - Basis for identification
NA
6 - Identified by
NA
7 - Original strain number
MG01
8 - Accession numbers in other collections
TBRC 15626
9 - Biological Safety Level
BSL-2
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Insect gut
14 - Category of substrate
Animal
15 - Locality
Amphawa canal, Amphawa District, Samut Songkhram Province
16 - Country of origin
Thailand
18 - Sample Collected by
NA
19 - Isolated by and date of isolation
Piyangkun Lueangjaroenkit, Feb. 2021
20 - Isolation details
NA
21 - Deposited by
Sasitorn Jindamorakot, Nov. 2021
22 - History
Sasitorn Jindamorakot > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
MYP
Title
Exophiala amphawaensis sp. nov., a novel black yeast isolated from the gut of amphipods in Thailand

Author

Tita Haranto, Pannida Khunnamwong, Koraon Wongkamhaeng, Piyangkun Lueangjaroenkit

Abstract

The genus Exophiala is polymorphic, able to transition between yeast, hyphal and pseudohyphal forms. Species of the genus Exophiala are ubiquitous fungi that are distributed in various environments around the world. During a survey of fungal diversity in the gut of amphipods (Floresorchestia amphawaensis and undescribed Dogielinotid amphipods) from the Amphawa estuary, Samut Songkhram province, Thailand, five black yeast strains (DMKU-MG01, DMKU-MG07, DMKU-MG08, DMKU-HG10 and DMKU-FG04) were identified as representing a novel taxon on the basis of a combination of morphological and molecular phylogenetic features. The five strains did not produce filamentous hyphae or pseudohyphae. Only budding yeast cells were observed. On the basis of the phenotypic characteristics and the results of molecular analyses of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, the five strains were identified as representing a novel species via applied nucleotide pairwise analysis. They differed from the most closely related species Exophiala alcalophiala by 3.54 % nucleotide substitutions (20 nucleotide substitutions in 572 bp) in the D1/D2 domains of the LSU rRNA gene. Moreover, the sequences of the ITS region of the five strains differed from those of the most closely related species E. alcalophiala, by 7.44–9.62 % nucleotide substitutions, and Exophiala halophiala, by 7.2–7.53 % nucleotide substitutions. The results of phylogenetic analyses based on the concatenated sequences of the ITS regions and the D1/D2 domains of the LSU rRNA gene confirmed that the five black yeast strains represented a single novel species of the genus Exophiala. In this study, Exophiala amphawaensis sp. nov. is proposed to accommodate these strains. The holotype is TBRC 15626T and the isotype is PYCC9020. The MycoBank accession number of the novel species is MB 851477.

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.006363
Publication Pdf