PYCC 8905
1 - Taxon name
Pichia bovicola
2 - Classification
Ascomycota
3 - Strain (species name) changes
Pichia sp.
4 - Status of the strain
Type strain of Pichia bovicola
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
Angchuan et al., 2022
7 - Original strain number
DMKU-MP2-6
8 - Accession numbers in other collections
TBRC 14828
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Pia (small intestital fluid of cattle)
14 - Category of substrate
Animal
15 - Locality
Pak Kret, Nonthaburi
16 - Country of origin
Thailand
18 - Sample Collected by
Unknown
19 - Isolated by and date of isolation
Jirameth Angchuan, Jan. 2021
20 - Isolation details
NA
21 - Deposited by
Sasitorn Jindamorakot, Apr. 2021
22 - History
Sasitorn Jindamorakot > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
Title
Pichia bovicola sp. nov., a yeast species associated with small-intestinal content of cattle

Author

Jirameth Angchuan, Varunya Sakpuntoon, Savitree Limtong, Nantana Srisuk

Abstract

Three yeast strains, DMKU-MP6-4T, DMKU-MP2-6 and DMKU-MP5-1, were isolated from the small-intestinal content or Pia of cattle in Thailand during the investigation of yeast diversity in this habitat. According to the D1/D2 domain of the large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) region sequence analysis, these strains represent a novel yeast species in the genus Pichia. The species produced one to four ascospores per ascus with spherical to ellipsoidal shape and heterogenous in terms of size. These three strains were identical and differed from their closely related species, Pichia exigua NRRL Y-10920T by 2% (six nucleotide substitutions and five gaps) in the D1/D2 domain of the large subunit rRNA gene, while ITS region sequences differed by 3.1% (16 nucleotide substitutions and 27 gaps), 3.7% (19 nucleotide substitutions and 28 gaps) and 3.1% (16 nucleotide substitutions and 27 gaps) for DMKU-MP6-4T, DMKU-MP2-6 and DMKU-MP5-1, respectively. The name, Pichia bovicola, is proposed to accommodate these species. The holotype is DMKU-MP6-4T (TBRC 15616T=PYCC 8905T).

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.005269