PYCC 8755
1 - Taxon name
Schizosaccharomyces osmophilus
2 - Classification
Ascomycota
3 - Strain (species name) changes
None
4 - Status of the strain
Type strain of Schizosaccharomyces osmophilus
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
NA
7 - Original strain number
SZ134-FG-A
8 - Accession numbers in other collections
NCAIM Y.02225T, CBS 15793T, CLIB 3267T
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Bee bread of Osmia florisomnis
14 - Category of substrate
Animal
15 - Locality
NA
16 - Country of origin
Germany
18 - Sample Collected by
NA
19 - Isolated by and date of isolation
NA
20 - Isolation details
NA
21 - Deposited by
Gábor Péter, Out. 2020
22 - History
Gábor Péter > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -80ºC
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
MY30G
DNA Sequence
Region
26S
Sequence Title
MK253005
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/MK253005
DNA Sequence
Region
ITS
Sequence Title
MK589403
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/MK589403
Title
Schizosaccharomyces osmophilus sp. nov., an osmophilic fission yeast occurring in bee bread of different solitary bee species

Author

Michael Brysch-Herzberg, Andrea Tobias, Martin Seidel, Rupert Wittmann, Elke Wohlmann, Reinhard Fischer, Dénes Dlauchy, Gabor Peter

Abstract

ABSTRACT Eight yeast strains that asexually reproduce by cell fission were isolated from bee bread of different solitary bees in Germany. DNA sequence analysis revealed that the strains shared the same sequence in the D1/D2 domain of the nuclear large subunit (LSU) rRNA gene with a strain that was previously isolated from a fig snack from Spain. The closest related type strain was that of Schizosaccharomyces octosporus, which showed 98.2% sequence similarity (11 substitutions) with the new strains. By clone sequence analysis of the internal transcribed spacer (ITS) region (ITS1, 5.8S rDNA, and ITS2) a total of nine different copy types were identified. The new strains differed from S. octosporus by approximately 31% in the ITS region. Sequence analysis of the RNAse P gene further supported the description of a new species. The strains isolated during this study show some phenotypic characteristics that separate them from the closest related species, S. octosporus and S. cryophilus. Since all strains showed true osmophily the name of the new species is S. osmophilus (holotype: CBS 15793T; isotype: CLIB 3267 T = NCAIM Y.02225 T, MycoBank no.: MB829586).

Publication Date

Link to Publication

https://doi.org/10.1093/femsyr/foz038
Publication Pdf