PYCC 8738

PYCC 8738
1 - Taxon name
Zygosaccharomyces gambellarensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Zygosaccharomyces gambellarensis
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
NA
7 - Original strain number
ZO03-5
8 - Accession numbers in other collections
CBS 12191T, MUCL 53393T
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
Yes
13 - Substrate of isolation
Wine (Vin Santo of Gambellara)
14 - Category of substrate
Food & beverages
15 - Locality
Gambellara, Vicenza
16 - Country of origin
Italy
17 - Latitude, longitude coordinates

45.4666666667, 11.3333333333

18 - Sample Collected by
NA
19 - Isolated by and date of isolation
M. Lorenzini and S. Torriani; Jan 2009
20 - Isolation details
NA
21 - Deposited by
G. Zapparoli, Out. 2020
22 - History
G. Zapparoli > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -80ºC
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
DNA Sequence
Region
26S
Sequence Title
FR725931
DNA Sequence
Region
16S
Sequence Title
FR725940
Title
Zygosaccharomyces gambellarensis sp. nov., an ascosporogenous yeast isolated from an Italian ‘passito’ style wine

Author

Sandra Torriani, Marilinda Lorenzini, Elisa Salvetti, Giovanna E. Felis

Abstract

Yeast strains were isolated from Vin Santo of Gambellara, a sweet white wine with the specificity of Controlled Designation of Origin produced from off-vine overripened grapes in the Veneto region (Italy). Comparative sequence analysis of the 26S rRNA gene revealed that three representative strains (ZO03-5T, CA06-8 and ME06-9) constitute a taxon related to, but distinct from,Zygosaccharomyces machadoi. Similarity between the 26S rRNA gene domain D1/D2 sequence of the three isolates andZ. machadoiwas 97.9 %; moreover, the morphological characteristics and the physiological behaviour also supported recognition of a novel taxon of osmophilic non-psychrophilic yeast showing a flower-like arrangement of budding cells that remain attached to each other. The nameZygosaccharomyces gambellarensisis proposed for the novel species, with ZO03-5T( = CBS 12191T = MUCL 53393T) as the type strain.

Publication Date

Link to Publication