PYCC 8719
1 - Taxon name
Nakazawaea siamensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Nakazawaea siamensis
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
NA
7 - Original strain number
DMKU-RK467
8 - Accession numbers in other collections
TBRC 1320; BCC 50734; CBS 12569; NBRC 108903
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
External surface of sugar cane leaf
14 - Category of substrate
Vascular plants, mosses & algae
15 - Locality
Nakhon Ratchasima province
16 - Country of origin
Thailand
18 - Sample Collected by
NA
19 - Isolated by and date of isolation
NA
20 - Isolation details
NA
21 - Deposited by
Sasitorn Jindamorakot, TBRC, Sept. 2020
22 - History
S. Jindamorakot > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -80ºC
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
DNA Sequence
Region
26S
Sequence Title
AB772177
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/AB772177
Title
Nakazawaea siamensis f.a., sp. nov., a yeast species isolated from phylloplane

Author

Rungluk Kaewwichian, Savitree Limtong

Abstract

Strain DMKU-RK467T, representing a novel yeast species, was isolated from the external surface of sugar cane leaves collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 region of the LSU rRNA gene and the internal transcribed spacer (ITS) region, strain DMKU-RK467T was assigned to a novel species of the genus Nakazawaea. The novel species was related most closely to the type strain of Candida wickerhamii but they differed by 1.9 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and by 5.2 % nucleotide substitutions in the ITS region. The name Nakazawaea siamensis f.a., sp. nov. is proposed (type strain DMKU-RK467T = BCC 50734T = NBRC 108903T = CBS 12569T).

Publication Date

Link to Publication

https://doi.org/10.1099/ijs.0.057521-0