PYCC 8718
1 - Taxon name
Kazachstania siamensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Kazachstania siamensis
5 - Basis for identification
Molecular (D1D2)
6 - Identified by
NA
7 - Original strain number
S-34
8 - Accession numbers in other collections
TBRC 640; BCC 21230; CBS 10361; NBRC 101968
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Soil
14 - Category of substrate
Soil
15 - Locality
Nakhon Ratchasima province
16 - Country of origin
Thailand
18 - Sample Collected by
NA
19 - Isolated by and date of isolation
NA
20 - Isolation details
NA
21 - Deposited by
Sasitorn Jindamorakot, TBRC, Sept. 2020
22 - History
S. Jindamorakot > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -80ºC
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
DNA Sequence
Region
26S
Sequence Title
AB258462
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/AB258462
Title
Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand

Author

Savitree Limtong, Wichien Yongmanitchai, Moe Moe Tun, Hiroko Kawasaki, Tatsuji Seki

Abstract

Two strains (S-34T and S-35) of a novel ascomycetous yeast species belonging to the genus Kazachstania were isolated from soil from a mixed deciduous forest in Amphoe Wang Nam Khiao, Nakhon Ratchasima Province, Thailand. The D1/D2 domains of the large-subunit rDNA sequences of the two strains were identical and also indicated a close relationship with respect to Kazachstania aquatica, Kazachstania unispora, Kazachstania aerobia, Kazachstania servazzii and Kazachstania solicola. The most closely related species, K. aquatica, has 14 nucleotide substitutions and three gaps in 566 nt. The phenotypic characteristics of the two strains were typical of those of members of the genus Kazachstania. These characteristics include the formation of a single globose ascospore in an unconjugated and persistent ascus, multilateral budding, the absence of arthrospores and ballistospores, the fermentation of glucose, the inability to assimilate nitrate, negative diazonium blue B and urease reactions, and the presence of ubiquinone Q-6. The novel strains can be distinguished from K. aquatica on the basis of a number of phenotypic characteristics and represent a novel species in the genus Kazachstania, for which the name Kazachstania siamensis sp. nov. is proposed. The type strain is S-34T (=CBS 10361T=NBRC 101968T=BCC 21230T).

Publication Date

Link to Publication

https://doi.org/10.1099/ijs.0.64539-0