PYCC 8655
1 - Taxon name
Metahyphopichia suwanaadthiae
2 - Classification
Ascomycota
3 - Strain (species name) changes
Maleea siamensis
4 - Status of the strain
Holotype of Metahyphopichia suwanaadthiae
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
Pannida Khunnamwong et. al. 2022
7 - Original strain number
DMKU-MRY16
8 - Accession numbers in other collections
NBRC 114386, TBRC 11775
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
unidentified mushroom
14 - Category of substrate
Fungi & yeasts
15 - Locality
Si Sa Ket province
16 - Country of origin
Thailand
18 - Sample Collected by
NA
19 - Isolated by and date of isolation
NA
20 - Isolation details
NA
21 - Deposited by
Savitree Limtong, Ago. 2020
22 - History
Savitree Limtong > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -80ºC
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
DNA Sequence
Region
ITS
Sequence Title
MT561458
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/1848663765
Title
Metahyphopichia suwanaadthiae sp. nov., an anamorphic yeast species in the order Saccharomycetales and reassignment of Candida silvanorum to the genus Metahyphopichia

Author

Pannida Khunnamwong, Ancharida Savarajara, Sasitorn Jindamorakot, Savitree Limtong

Abstract

Seven yeast strains, representing a single novel anamorphic species, were isolated in Thailand. They consisted of five strains (DMKU-MRY16T, DMKU-SK18, DMKU-SK25, DMKU-SK30 and DMKU-SK32) obtained from five different mushrooms, and two strains (ST-224 and 11-14.2) derived from insect frass and soil, respectively. The pairwise sequence analysis indicated that all seven strains had identical sequences in the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region. Metahyphopichia silvanorum was the most closely related species, but with 11.9–12.4% nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 13.1–13.3% nucleotide substitutions in the ITS region. The phylogenetic analyses based on the concatenated sequences of the ITS region and the D1/D2 domains of the LSU rRNA gene showed that the seven strains form a well-separated subclade in a clade containing M. silvanorum and Metahyphopichia laotica with high bootstrap support. A phylogenetic analysis of a multilocus dataset including the small subunit (SSU) rRNA gene, the ITS region, the D1/D2 domains of the LSU rRNA gene, translation elongation factor 1-alpha gene, actin gene and the RNA polymerase II subunit 2 gene, confirmed the presence of the monophyletic clade that also includes M. silvanorum and M. laotica, and strongly supported the phylogenetic isolation of the seven strains from its neighbouring species. Therefore, the seven strains were assigned as a single novel species of the genus Metahyphopichia, according to their phylogenetic relationships. The name Metahyphopichia suwanaadthiae sp. nov. is proposed to accommodate the seven strains. The holotype is DMKU-MRY16T (TBRC 11775T=NBRC 114386T=PYCC 8655T). The MycoBank number of the novel species is MB 841280. In addition, Candida silvanorum is reassigned to the genus Metahyphopichia. The MycoBank number of M. silvanorum comb. nov. is MB 841279.

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.005183