PYCC 3026

General Information

Taxonomy

1 - Taxon name

Candidozyma haemuli

2 - Classification

Ascomycota

3 - Strain (species name) changes

Torulopsis haemulonii; Candida haemulonii

4 - Status of the strain

Other Collections

8 - Accession numbers in other collections

CBS 5150

Strain details

11 - PYCC strain status

Open

12 - Mediterranean strain

Yes

13 - Substrate of isolation

sea water

14 - Category of substrate

Water

15 - Locality

off Lisbon

16 - Country of origin

Portugal

19 - Isolated by and date of isolation

N. van Uden & Kolipinski, 1961

21 - Deposited by

N. van Uden, 1963

23 - Preservation

Glass beads; 20% Glycerol; -150ºC

24 - Price per culture

90€

Growth conditions

26 - Medium for growth

YMA

Molecular data

DNA Sequence

Region

26S

Sequence Title

MZ340603

Gene Bank Url

https://www.ncbi.nlm.nih.gov/nuccore/MZ340603

Phenotype

Fermentation

F1 D-Glucose

F2 D-Galactose

F3 Maltose

F5 Sucrose

F6 a,a-Trehalose

W, D

F7 Melibiose

F8 Lactose

F9 Cellobiose

F10 Melezitose

F11 Raffinose

W, D

F12 Inulin

Assimilation-Growth

C1 D-Glucose

C2 D-Galactose

C3 L-Sorbose

C4 D-Glucosamine

C5 D-Ribose

C6 D-Xylose

C7 L-Arabinose

C8 D-Arabinose

C9 L-Rhamnose

C10 Sucrose

C11 Maltose

C12 a,a-Trehalose

C13 Me a-D-Glucoside

C14 Cellobiose

C15 Salicin

C16 Arbutin spliting

C17 Melibiose

C18 Lactose

C19 Raffinose

C20 Melezitose

C21 Inulin

C22 Starch

C23 Glycerol

C24 Erythritol

C25 Ribitol

C26 Xylitol

C28 D-Glucitol

C29 D-Mannitol

C30 Galactitol

C31 myo-Inositol

C32 D-Glucono-1,5-lactone

C35 D-Gluconate

C36 D-Glucuronate

C38 DL-Lactate

C39 Succinate

C40 Citrate

C41 Methanol

C42 Ethanol

C50 L-Malic acid

C51 L-Tartaric acid

N1 Nitrate

N2 Nitrite

N3 Ethylamine

N4 L-Lysine

N5 Cadaverine

N6 Creatine

N7 Creatinine

V1 w/o vitamins

T1 Growth at 25ºC

T1 Growth at 30ºC

T3 Growth at 35ºC

T4 Growth at 37ºC

T5 Growth at 40ºC

O1 Cycloheximide 0.01%

O2 Cycloheximide 0.1%

O4 50% D-Glucose

O6 10% NaCl

M1 Starch formation

M3 Urea hydrolysis

M4 Diazonium Blue B reaction

Bibliography

Title

Phylogenomic analysis of the Candida auris- Candida haemuli clade and related taxa in the Metschnikowiaceae, and proposal of thirteen new genera, fifty-five new combinations and nine new species

Author

F. Liu, Z.-D. Hu, X.-M. Zhao, W.-N. Zhao, Z.-X. Feng, A. Yurkov, S. Alwasel, T. Boekhout, K. Bensch, F.-L. Hui, F.-Y. Bai, Q.-M. Wang

Abstract

Candida is a polyphyletic genus of sexually reproducing yeasts in the Saccharomycotina with more than 400 species that occur in almost all families of the subclass and its name is strongly connected with the infectious disease candidiasis. During the last two decades, approximately half of the Candida species have been reassigned into more than 36 already existing genera and 14 newly proposed genera, but the polyphyletic feature of the genus largely remained. Candida auris is an important, globally emerging opportunistic pathogen that has caused life-threatening outbreaks in healthcare facilities worldwide. This species belongs to the Candida auris - Candida haemuli (CAH) clade in the Metschnikowiaceae, a clade that contains multidrug-resistant clinically relevant species, but also species isolated from natural environments. The clade is phylogenetically positioned remotely from the type species of the genus Candida that is Candida vulgaris (currently interpreted as a synonym of Candida tropicalis) and belongs to the family Debaryomycetaceae. Although previous phylogenetic and phylogenomic studies confirmed the position of C. auris in the Metschnikowiaceae, these analyses failed to resolve the position of the CAH clade within the family and its delimitation from the genera Clavispora and Metschnikowia. To resolve the position of the CAH clade, phylogenomic and comparative genomics analyses were carried out to address the phylogenetic position of C. auris and related species in the Metschnikowiaceae using several metrics, such as the average amino acid identity (AAI) values, the percentage of conserved proteins (POCP), and the presence-absence patterns of orthologs (PAPO).Based on those approaches, 13 new genera are proposed for various Candida and Hyphopichia species, including members of the CAH clade in the Metschnikowiaceae. As a result, C. auris and related species are reassigned to the genus Candidozyma. Fifty-five new combinations and nine new species are introduced, and this will reduce the polyphyly of the genus Candida.

Publication Date

07 Apr 2024

Link to Publication

https://doi.org/10.3767/persoonia.2024.52.02