PYCC 3025
1 - Taxon name
Candidozyma haemuli
2 - Classification
Ascomycota
3 - Strain (species name) changes
Torulopsis haemulonii; Candida haemulonii; Candida haemulonis
4 - Status of the strain
Type strain of Candidozyma haemuli
7 - Original strain number
MI 37
8 - Accession numbers in other collections
ATCC 22991;CBS 5149;DBVPG 6958;IFO 10001;JCM 3762;NRRL Y-6693
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
gut of fish Haemulon sciurus (blue-striped grunt fish)
14 - Category of substrate
Animal
15 - Locality
Florida, off Miami
16 - Country of origin
USA
19 - Isolated by and date of isolation
N. van Uden
21 - Deposited by
N. van Uden, 1963
23 - Preservation
Glass beads; 20% Glycerol; -150ºC Freeze dried ampoules
24 - Price per culture
90€
26 - Medium for growth
YMA
DNA Sequence
Region
ITS
Sequence Title
AY500375
Gene Bank Url
http://www.ncbi.nlm.nih.gov/nuccore/AY500375
DNA Sequence
Region
16S
Sequence Title
AJ606467
Gene Bank Url
http://www.ncbi.nlm.nih.gov/nuccore/AJ606467
DNA Sequence
Region
16S
Sequence Title
AB013572
Gene Bank Url
http://www.ncbi.nlm.nih.gov/nuccore/AB013572
F1 D-Glucose
+
F2 D-Galactose
-
F3 Maltose
-
F5 Sucrose
W, D
F6 a,a-Trehalose
W, D
F7 Melibiose
-
F8 Lactose
-
F9 Cellobiose
-
F10 Melezitose
-
F11 Raffinose
-
F12 Inulin
-
C1 D-Glucose
+
C2 D-Galactose
W
C3 L-Sorbose
-
C4 D-Glucosamine
+
C5 D-Ribose
+
C6 D-Xylose
W
C7 L-Arabinose
-
C8 D-Arabinose
-
C9 L-Rhamnose
+
C10 Sucrose
+
C11 Maltose
+
C12 a,a-Trehalose
+
C13 Me a-D-Glucoside
-
C14 Cellobiose
-
C15 Salicin
-
C16 Arbutin spliting
+
C17 Melibiose
-
C18 Lactose
-
C19 Raffinose
+
C20 Melezitose
+
C21 Inulin
-
C22 Starch
-
C23 Glycerol
+
C24 Erythritol
-
C25 Ribitol
-
C28 D-Glucitol
+
C29 D-Mannitol
+
C30 Galactitol
+
C31 myo-Inositol
-
C32 D-Glucono-1,5-lactone
+
C35 D-Gluconate
+
C36 D-Glucuronate
-
C38 DL-Lactate
-
C39 Succinate
+
C40 Citrate
+
C41 Methanol
-
C42 Ethanol
W, D +
C50 L-Malic acid
+
C51 L-Tartaric acid
-
N1 Nitrate
-
N2 Nitrite
-
N3 Ethylamine
+
N4 L-Lysine
+
N5 Cadaverine
+
N6 Creatine
-
N7 Creatinine
-
V1 w/o vitamins
-
T1 Growth at 25ºC
+
T1 Growth at 30ºC
+
T3 Growth at 35ºC
+
T4 Growth at 37ºC
+
T5 Growth at 40ºC
-
O1 Cycloheximide 0.01%
+
O2 Cycloheximide 0.1%
W
O4 50% D-Glucose
+
O6 10% NaCl
+
M1 Starch formation
-
M3 Urea hydrolysis
-
M4 Diazonium Blue B reaction
-
Title
Phylogenomic analysis of the Candida auris- Candida haemuli clade and related taxa in the Metschnikowiaceae, and proposal of thirteen new genera, fifty-five new combinations and nine new species

Author

F. Liu, Z.-D. Hu, X.-M. Zhao, W.-N. Zhao, Z.-X. Feng, A. Yurkov, S. Alwasel, T. Boekhout, K. Bensch, F.-L. Hui, F.-Y. Bai, Q.-M. Wang

Abstract

Candida is a polyphyletic genus of sexually reproducing yeasts in the Saccharomycotina with more than 400 species that occur in almost all families of the subclass and its name is strongly connected with the infectious disease candidiasis. During the last two decades, approximately half of the Candida species have been reassigned into more than 36 already existing genera and 14 newly proposed genera, but the polyphyletic feature of the genus largely remained. Candida auris is an important, globally emerging opportunistic pathogen that has caused life-threatening outbreaks in healthcare facilities worldwide. This species belongs to the Candida auris - Candida haemuli (CAH) clade in the Metschnikowiaceae, a clade that contains multidrug-resistant clinically relevant species, but also species isolated from natural environments. The clade is phylogenetically positioned remotely from the type species of the genus Candida that is Candida vulgaris (currently interpreted as a synonym of Candida tropicalis) and belongs to the family Debaryomycetaceae. Although previous phylogenetic and phylogenomic studies confirmed the position of C. auris in the Metschnikowiaceae, these analyses failed to resolve the position of the CAH clade within the family and its delimitation from the genera Clavispora and Metschnikowia. To resolve the position of the CAH clade, phylogenomic and comparative genomics analyses were carried out to address the phylogenetic position of C. auris and related species in the Metschnikowiaceae using several metrics, such as the average amino acid identity (AAI) values, the percentage of conserved proteins (POCP), and the presence-absence patterns of orthologs (PAPO).Based on those approaches, 13 new genera are proposed for various Candida and Hyphopichia species, including members of the CAH clade in the Metschnikowiaceae. As a result, C. auris and related species are reassigned to the genus Candidozyma. Fifty-five new combinations and nine new species are introduced, and this will reduce the polyphyly of the genus Candida.

Publication Date

Link to Publication

https://doi.org/10.3767/persoonia.2024.52.02