PYCC 8490
1 - Taxon name
Savitreea pentosicarens
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Savitreea pentosicarens
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
Varunya Sakpuntoon
7 - Original strain number
DMKU-GTCP10-8
8 - Accession numbers in other collections
TBRC 12159
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Grease trap of Kasetsart University's canteen
14 - Category of substrate
Other
15 - Locality
Chatuchak, Bangkok
16 - Country of origin
Thailand
17 - Latitude, longitude coordinates

13, 100

18 - Sample Collected by
NA
19 - Isolated by and date of isolation
Jiremeth Angchuan
20 - Isolation details
direct isolation in YPD
21 - Deposited by
Nantana Srisuk, Feb. 2020
22 - History
Nantana Srisuk > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -80ºC
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
DNA Sequence
Region
ITS
Sequence Title
MN977919
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/MN977919
DNA Sequence
Region
26S
Sequence Title
MN966866
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/MN966866
Title
Savitreea pentosicarens gen. nov., sp. nov., a yeast species in the family Saccharomycetaceae isolated from a grease trap

Author

Varunya Sakpuntoon, Jirameth Angchuan, Chanita Boonmak, Pannida Khunnamwong, Noémie Jacques, Cécile Grondin, Serge Casaregola, Nantana Srisuk

Abstract

Two strains (DMKU-GTCP10-8 and CLIB 1740) representing a novel anamorphic yeast species were isolated from a grease sample collected from a grease trap in Thailand and from an unidentified fungus collected in French Guiana, respectively. On the basis of phylogenetic analysis based on the combined D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, Lachancea fermentati CBS 707T was the closely related species with 12.8 % sequence divergence (70 nucleotide substitutions and three gaps in 571 nucleotides) and 28.1 % sequence divergence (93 nucleotide substitutions and 90 gaps in 651 nucleotides) in the D1/D2 domain of the LSU rRNA gene and the ITS region, respectively. Phylogenetic analysis based on the concatenated sequences of the five genes including the small subunit rRNA gene, the D1/D2 domain of the LSU rRNA gene, the ITS region, translation elongation factor-1 alpha (TEF1) and RNA polymerase II subunit 2 (RPB2) genes confirmed that the two strains (DMKU-GTCP10-8 and CLIB 1740) were well-separated from other described yeast genera in Saccharomycetaceae. Hence, Savitreea pentosicarens gen. nov., sp. nov. is proposed to accommodate these two strains as members of the family Saccharomycetaceae. The holotype is S. pentosicarens DMKU-GTCP10-8T (ex-type strain TBRC 12159=PYCC 8490; MycoBank number 835044).

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.004457