PYCC 8459
1 - Taxon name
Diutina siamensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Diutina siamensis
5 - Basis for identification
Molecular (D1D2)
6 - Identified by
Sasitorn Jindamorakot
7 - Original strain number
DMKU-RE43
8 - Accession numbers in other collections
BCC 61183; CBS 13388; NBRC 109695; TBRC 4476
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
Leaves of rice
14 - Category of substrate
Vascular plants, mosses & algae
15 - Locality
Suphan Buri Province, U Thong Destrict
16 - Country of origin
Thailand
18 - Sample Collected by
Pannida and and Savitree Limtong
19 - Isolated by and date of isolation
Kanya Kokaew & Savitree Limtong
20 - Isolation details
NA
21 - Deposited by
Sasitorn Jindamorakot, TBRC, Jan. 2020
22 - History
S. Jindamorakot > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -80ºC
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
DNA Sequence
Region
ITS
Sequence Title
KT336715
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/927348142
Title
Description of Diutina gen. nov., Diutina siamensis, f.a. sp. nov., and reassignment of Candida catenulata, Candida mesorugosa, Candida neorugosa, Candida pseudorugosa, Candida ranongensis, Candida rugosa and Candida scorzettiae to the genus Diutina

Author

Pannida Khunnamwong, Noppon Lertwattanasakul, Sasitorn Jindamorakot, Savitree Limtong, Marc-André Lachance

Abstract

Three strains (DMKU-RE28, DMKU-RE43T and DMKU-RE123) of a novel anamorphic yeast species were isolated from rice leaf tissue collected in Thailand. DNA sequence analysis demonstrated that the species forms a sister pair with Candida ranongensis CBS 10861T but differs by 24–30 substitutions in the LSU rRNA gene D1/D2 domains and 30–35 substitutions in the ITS region. A phylogenetic analysis based on both the small and the large rRNA gene subunits confirmed this connection and demonstrated the presence of a clade that also includes Candida catenulata, Candida mesorugosa, Candida neorugosa, Candida pseudorugosa, Candida rugosa and Candida scorzettiae. The clade is not closely affiliated to any known teleomorphic genus, and forms a well-separated lineage from currently recognized genera of the Saccharomycetales. Hence, the genus Diutina gen. nov. is proposed to accommodate members of the clade, including Diutina siamensis f.a. sp. nov. and the preceding seven Candida species. The type strain is DMKU-RE43T ( = CBS 13388T = BCC 61183T = NBRC 109695T).

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.000634