PYCC 10335
1 - Taxon name
Metschnikowia sanitii
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Metschnikowia sanitii
5 - Basis for identification
Molecular (whole genome sequence)
6 - Identified by
Maneerat Khammeankea et. al. 2026
7 - Original strain number
DMKU-FF11
8 - Accession numbers in other collections
TBRC 19861
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
floral surface of mangrove trees
14 - Category of substrate
Vascular plants, mosses & algae
15 - Locality
Mangrove Botanical Garden, Mueang Chanthaburi, Chanthaburi
16 - Country of origin
Thailand
18 - Sample Collected by
NA
19 - Isolated by and date of isolation
Pannida Khunnamwong, 12/06/2023
20 - Isolation details
NA
21 - Deposited by
Sasitorn Jindamorakot, Apr. 2025
22 - History
Sasitorn Jindamorakot > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
90€
25 - Remarks
NA
26 - Medium for growth
YMA
Whole genome
SRX31323031
Title
Metschnikowia sanitii f.a., sp. nov., a new ascomycetous yeast species isolated from mangrove forests in Thailand

Author

Maneerat Khammeankea, Pajongwan Gungprakhon, Chakrit Jiarasatit, Naree Jittisamont, Sasitorn Jindamorakot, Somjit Am-In, Thanasak Lomthong, Savitree Limtong, Sorawee Kaewkarn, Arissara Tubtimyoy, Worarat Kruasuwan, Pannida Khunnamwong

Abstract

Seven strains representing a novel ascomycetous yeast species were isolated from the leaf phylloplane (DMKU-SG60) and the floral surface (DMKU-FF11, DMKU-FF12, DMKU-FD2, DMKU-FD6, DMKU-FS1 and ST-387) of mangrove trees in Thailand. Pairwise sequence analysis indicated that the strains were identical or differed by one to two nucleotide substitution(s) in the D1/D2 domains of the large subunit (LSU) rRNA gene. They also were identical or differed by one to four nucleotide substitution(s) in the internal transcribed spacer (ITS) region. blast n searches of the GenBank database revealed that the closest related species was Metschnikowia rancensis , differing by 28 (5.5–6.0%) and 30–33 (8.0–8.9%) nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and the ITS region, respectively. Moreover, whole-genome sequencing was used to further confirm the separation of this species. In comparison with other related species, average nucleotide identity values of 74.6–76.5% and digital DNA–DNA hybridization values of 12.8–13.3% were obtained. Phylogenetic analysis based on the concatenated sequences of the ITS region and the D1/D2 domains of the LSU rRNA gene, as well as phylogenomic analysis, showed that these strains belong to the genus Metschnikowia , but at a distinct position relative to other recognized species. Based on molecular analyses and phenotypic characteristics, the strains represent a novel species, for which the name Metschnikowia sanitii f.a., sp. nov. is proposed. The holotype is TBRC 19861 T , and the ex-type culture is PYCC 10335 (DMKU-FF11). The MycoBank number is MB 860254.

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.007041
Publication Pdf