PYCC 10067
1 - Taxon name
Hyphopichia xiaguanensis
2 - Classification
Ascomycota
3 - Strain (species name) changes
NA
4 - Status of the strain
Type strain of Hyphopichia xiaguanensis
5 - Basis for identification
Molecular (D1D2 & ITS)
6 - Identified by
Wen-Ting Hu at. al. 2022
7 - Original strain number
NYNU 20899
8 - Accession numbers in other collections
CBS 16668
9 - Biological Safety Level
BSL-1
10 - Access and Benefit Sharing (CBD, Nagoya protocol)
No known ABS restrictions
11 - PYCC strain status
Open
12 - Mediterranean strain
No
13 - Substrate of isolation
plant leaves
14 - Category of substrate
Vascular plants, mosses & algae
15 - Locality
Baotianman Nature Reserve in Henan Province
16 - Country of origin
China
18 - Sample Collected by
NA
19 - Isolated by and date of isolation
NA
20 - Isolation details
YMA with chloramphenicol, 20ºC
21 - Deposited by
Feng-Li, Nov. 2024
22 - History
Fen-Li > PYCC
23 - Preservation
Glass beads; 20% Glycerol; -150C
24 - Price per culture
85€
25 - Remarks
NA
26 - Medium for growth
YMA
DNA Sequence
Region
26S
Sequence Title
MW362351
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/MW362351
DNA Sequence
Region
ITS
Sequence Title
MW362352
Gene Bank Url
https://www.ncbi.nlm.nih.gov/nuccore/MW362352
Title
Hyphopichia xiaguanensis f.a., sp. nov., an ascomycetous yeast species isolated from plant leaves

Author

Wen-Ting Hu, Shuai-Bei Chu, Ying Li, Feng-Li Hui

Abstract

Two strains representing a novel yeast species were isolated from plant leaves collected in the Baotianman Nature Reserve in Henan Province, central China. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) region (ITS1-5.8S–ITS2) and the D1/D2 domain of the large subunit rRNA gene revealed that the novel species belonged to the genus Hyphopichia, although the formation of ascospores was not observed. The novel species was related most closely to Hyphopichia paragotoi CBS 13913T but they differed by 0.9 % sequence divergence (five substitutions) in the D1/D2 domain and by 3.7 % sequence divergence (seven substitutions and eight gaps) in the ITS region. Furthermore, the novel species can also be differentiated from the closely related species in some biochemical and physiological characteristics. The species name of Hyphopichia xiaguanensis f.a., sp. nov. (Holotype CBS 16668, Mycobank MB 842425) is proposed to accommodate strains NYNU 20899T and NYNU 20914.

Publication Date

Link to Publication

https://doi.org/10.1099/ijsem.0.005398